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Research at NANUC

Papers Acknowledging NANUC


Overduin M, Esmaili M. Structures and Interactions of Transmembrane Targets in Native Nanodiscs. SLAS Discov. in press.

Barr A, Overduin M. Protein-Protein Interactions. J Vis Exp. 148. doi: 10.3791/59816.

Overduin M, Esmaili M. Memtein: The fundamental unit of membrane-protein structure and function. Chem Phys Lipids.218:73-84.

Eitzen G, Smithers CC, Murray AG, Overduin M. Structure and function of the Fgd family of divergent FYVE domain proteins.. Biochem Cell Biol. 97(3):257-264.

Overduin M, Esmaili M. Converging lipidomics, metabolomics, interactomics and proteomics within native nanodiscs. Applied Sciences, 9(6), 1230

Ouyang Z, Felix J, Zhou J, Pei Y, Ma B, Hwang PM, Lemieux MJ, Gutsche I, Zheng F, Wen Y. Trimeric structure of the mouse Kupffer cell C-type lectin receptor Clec4f. FEBS press.

Hwang P, Mingels A, Kavsak PA. High-sensitivity cardiac troponin testing during and after ACS: Complexed or not? Clin Biochem. in press.

Mahmud Z, Zahran S, Liu PB, Reiz B, Chan BYH, Roczkowsky A, McCartney CE, Davies PL, Li L, Schulz R, Hwang PM. Structure and proteolytic susceptibility of the inhibitory C-terminal tail of cardiac troponin I. Biochim Biophys Acta 1863(4):661-671.

Tikunova SB, Cuesta A, Price M, Li MX, Belevych N, Biesiadecki BJ, Reiser PJ, Hwang PM, Davis JP. 3-Chlorodiphenylamine activates cardiac troponin by a mechanism distinct from bepridil or TFP. J Gen Physiol. 151(1):9-17.

Klein BA, Sykes BD. Feasibility of trifluoromethyl TROSY NMR at high magnetic fields. J Biomol NMR

Fu ZL, Holmes PC, Westaway D, Sykes BD. Nascent β Structure in the Elongated Hydrophobic Region of a Gerstmann-Sträussler-Scheinker PrP Allele. J Mol Biol. 431(14):2599-2611.

Wong KY, McKay R, Liu Y, Towle K, Elloumi Y, Li X, Quan S, Dutta D, Sykes BD, Fliegel L. Diverse residues of intracellular loop 5 of the Na(+)/H(+) exchanger modulate proton sensing, expression, activity and targeting. Biochim Biophys Acta Biomembr. 1861(1):191-200.

Rashid S, Lee BL, Wajda B, Spyracopoulos L. Side-Chain Dynamics of the Trifluoroacetone Cysteine Derivative Characterized by (19)F NMR Relaxation and Molecular Dynamics Simulations. J Phys Chem B. 123(17):3665-3671.

Lee BL, Rashid S, Wajda B, Wolmarans A, LaPointe P, Spyracopoulos L. The Hsp90 Chaperone: (1)H and (19)F Dynamic Nuclear Magnetic Resonance Spectroscopy Reveals a Perfect Enzyme. Biochemistry. 58(14):1869-1877.

Markin CJ, Mercier P, Spyracopoulos L. Role of Polyubiquitin Chain Flexibility in the Catalytic Mechanism of Cullin-RING Ubiquitin Ligases. J Phys Chem B. 123(4):776-786.

Dong C, Garen CR, Mercier P, Petersen NO, Woodside MT. Characterizing the inhibition of α-synuclein oligomerization by a pharmacological chaperone that prevents prion formation by the protein PrP. Protein Sci. 8(9):1690-1702.


G Winkelaar, C Trieber, J Kumar, M Overduin. 2018. Measuring interactions of globular and filamentous proteins by nuclear magnetic resonance spectroscopy (NMR) and microscale thermophoresis (MST), JoVE, 141. doi: 10.3791/58537.

T Mrozowich, S McLennan, M Overduin, T Patel. 2018. Structural Studies of Macromolecular Interactions in Solution using Small Angle X-Ray Scattering, JoVE, 141. doi: 10.3791/58538

B Klumperman, M. Overduin. 2018. Advancing membrane biology with poly(styrene-co-maleic acid)-based native nanodiscs, Eur Polym J 110, 63-68

MJ Geer, A van Geffen, P Gopalasingam, T Vögtle, CW Smith, S Heising, MJE Kuijper, BME Tullemans, GE Jarvis, JA Eble, M Jeeves, M Overduin, JW Heemskerk, A Mazharian, YA Senis Uncoupling of the ITIM receptor G6b-B from the tyrosine phosphatases Shp1 and Shp2 disrupts platelet homeostasis in mice, Blood, 132(13):1413-1425

Lenoir M, Ustunel C, Rajesh S, Kaur J, Moreau D, Gruenberg J, Overduin M.Phosphorylation of conserved phosphoinositide binding pocket regulates sorting nexin membrane targeting. Nat Commun. 9(1):993.

El Turk F, De Genst E, Guilliams T, Fauvet B, Hejjaoui M, Di Trani J, Chiki A, Mittermaier A, Vendruscolo M, Lashuel HA, Dobson CM. Exploring the role of post-translational modifications in regulating α-synuclein interactions by studying the effects of phosphorylation on nanobody binding. Protein Sci. in press.

Harris J, Shadrina M, Oliver C, Vogel J, Mittermaier A. Concerted millisecond timescale dynamics in the intrinsically disordered carboxyl terminus of γ-tubulin induced by mutation of a conserved tyrosine residue.Protein Sci. 27(2):531-545.

Rout MK, Lee BL, Lin A, Xiao W, Spyracopoulos L. Active Site Gate Dynamics Modulate the Catalytic Activity of the Ubiquitination Enzyme E2-25K. Sci Rep. 3;8(1):7002.

Ali MAM, Strickfaden H, Lee BL, Spyracopoulos L, Hendzel MJ. RYBP Is a K63-Ubiquitin-Chain-Binding Protein that Inhibits Homologous Recombination Repair. Cell Rep. 22(2):383-395.

Di Trani JM, De Cesco S, O'Leary R, Plescia J, do Nascimento CJ, Moitessier N, Mittermaier AK. Rapid measurement of inhibitor binding kinetics by isothermal titration calorimetry. Nat Commun. 9(1):893.

Klein BA, Reiz B, Robertson IM, Irving M, Li L, Sun YB, Sykes BD. Reversible Covalent Reaction of Levosimendan with Cardiac Troponin C in Vitro and in Situ. Biochemistry. 57(15):2256-2265.

Mercer RCC, Daude N, Dorosh L, Fu ZL, Mays CE, Gapeshina H, Wohlgemuth SL, Acevedo-Morantes CY, Yang J, Cashman NR, Coulthart MB, Pearson DM, Joseph JT, Wille H, Safar JG, Jansen GH, Stepanova M, Sykes BD, Westaway D. A novel Gerstmann-Sträussler-Scheinker disease mutation defines a precursor for amyloidogenic 8 kDa PrP fragments and reveals N-terminal structural changes shared by other GSS alleles. PLoS Pathog. 14(1):e1006826.

Esmaili M and Overduin M. Membrane biology visualized in nanometer-sized discs formed by styrene maleic acid polymers, Biochim Biophys Acta.1860(2):257-263.


Lee BL, Singh A, Mark Glover JN, Hendzel MJ, Spyracopoulos L. Molecular Basis for K63-Linked Ubiquitination Processes in Double-Strand DNA Break Repair: A Focus on Kinetics and Dynamics. J Mol Biol. 429(22):3409-3429.

Danmaliki GI, Liu PB, Hwang PM. Stereoselective Deuteration in Aspartate, Asparagine, Lysine, and Methionine Amino Acid Residues Using Fumarate as a Carbon Source for Escherichia coli in D2O. Biochemistry. 56(45):6015-6029.

Diviani D, Raimondi F, Del Vescovo CD, Dreyer E, Reggi E, Osman H, Ruggieri L, Gonano C, Cavin S, Box CL, Lenoir M, Overduin M, Bellucci L, Seeber M, Fanelli F. Small-Molecule Protein-Protein Interaction Inhibitor of Oncogenic Rho Signaling. Cell Chem Biol. (16)30245-8.

Smithers CC, Overduin M. Structural Mechanisms and Drug Discovery Prospects of Rho GTPases. Cells. 5(2).

Fogl C, Mohammed F, Al-Jassar C, Jeeves M, Knowles TJ, Rodriguez-Zamora P, White SA, Odintsova E, Overduin M, Chidgey M. Mechanism of intermediate filament recognition by plakin repeat domains revealed by envoplakin targeting of vimentin. Nature Commun. 7:10827.

Guix FX, Sannerud R, Berditchevski F, Arranz AM, Horré K, Snellinx A, Thathiah A, Saido T, Saito T, Rajesh S, Overduin M, Kumar-Singh S, Radaelli E, Corthout N, Colombelli J, Tosi S, Munck S, Salas IH, Annaert W, De Strooper B. Tetraspanin 6: a pivotal protein of the multiple vesicular body determining exosome release and lysosomal degradation of amyloid precursor protein fragments.Mol Neurodegener 10;12(1):25.

Bédard M, Roy V, Montagne M, Lavigne P. Structural Insights into c-Myc-interacting Zinc Finger Protein-1 (Miz-1) Delineate Domains Required for DNA Scanning and Sequence-specific Binding. J Biol Chem 292(8):3323-3340.

Huang SK, Shin K, Sarker M, Rainey JK. Apela exhibits isoform- and headgroup-dependent modulation of micelle binding, peptide conformation and dynamics. Biochim Biophys Acta 1859(5):767-778.


Diviani D, Raimondi F, Del Vescovo CD, Dreyer E, Reggi E, Osman H, Ruggieri L, Gonano C, Cavin S, Box CL, Lenoir M, Overduin M, Bellucci L, Seeber M, Fanelli F. Small-Molecule Protein-Protein Interaction Inhibitor of Oncogenic Rho Signaling. Cell Chem Biol. (16)30245-8.

Sarker M, Orrell KE, Xu L, Tremblay ML, Bak JJ, Liu XQ, Rainey JK. Tracking Transitions in Spider Wrapping Silk Conformation and Dynamics by (19)F Nuclear Magnetic Resonance Spectroscopy. Biochemistry. 55(21):3048-59.

Pandey A, Shin K, Patterson RE, Liu XQ, Rainey JK. Current strategies for protein production and purification enabling membrane protein structural biology. Biochem Cell Biol. 94(6):507-527.

Wolmarans A, Lee B, Spyracopoulos L, LaPointe P. The Mechanism of Hsp90 ATPase Stimulation by Aha1. Sci Rep. 6:33179.

Spyracopoulos L. The Proteasome: More Than a Means to an End. Structure. 24(8):1221-3.

Hodge CD, Spyracopoulos L, Glover JN. Ubc13: the Lys63 ubiquitin chain building machine. Oncotarget. 2016 Sep 27;7(39):64471-64504.

Anamika, Spyracopoulos L. Molecular Basis for Phosphorylation-dependent SUMO Recognition by the DNA Repair Protein RAP80. J Biol Chem. 2016 Feb 26;291(9):4417-28.

Cai F, Li MX, Pineda-Sanabria SE, Gelozia S, Lindert S, West F, Sykes BD, Hwang PM. Structures reveal details of small molecule binding to cardiac troponin. J Mol Cell Cardiol. 101:134-144. 1;

Robertson IM, Pineda-Sanabria SE, Yan Z, Kampourakis T, Sun YB, Sykes BD, Irving M. Reversible Covalent Binding to Cardiac Troponin C by the Ca(2+)-Sensitizer Levosimendan. Biochemistry 55(43):6032-6045.

Assi HA, Harkness RW 5th, Martin-Pintado N, Wilds CJ, Campos-Olivas R, Mittermaier AK, González C, Damha MJ. Stabilization of i-motif structures by 2'-β-fluorination of DNA. Nucleic Acids Res. 44(11):4998-5009.

Harkness RW 5th, Mittermaier AK. G-register exchange dynamics in guanine quadruplexes. Nucleic Acids Res. 44(8):3481-94. .

Gräβel L, Fast LA, Scheffer KD, Boukhallouk F, Spoden GA, Tenzer S, Boller K, Bago R, Rajesh S, Overduin M, Berditchevski F, Florin L. The CD63-Syntenin-1 Complex Controls Post-Endocytic Trafficking of Oncogenic Human Papillomaviruses. Sci Rep. 6:32337.

Smithers CC, Overduin M. Structural Mechanisms and Drug Discovery Prospects of Rho GTPases. Cells. 5(2).

Fogl C, Mohammed F, Al-Jassar C, Jeeves M, Knowles TJ, Rodriguez-Zamora P, White SA, Odintsova E, Overduin M, Chidgey M. Mechanism of intermediate filament recognition by plakin repeat domains revealed by envoplakin targeting of vimentin. Nature Commun. 7:10827.


Li MX, Hwang PM. Structure and function of cardiac troponin C (TNNC1):Implications for heart failure, cardiomyopathies, and troponin modulating drugs. Gene. 571:153-66.

Hwang PM, Sykes BD. Targeting the sarcomere to correct muscle function. Nat Rev Drug Discov. 14(5):313-28.

Lenoir M, Kufareva I, Abagyan R, Overduin M. Membrane and Protein Interactions of the Pleckstrin Homology Domain Superfamily. Membranes. 5:646-63.

Jamshad M, Charlton J, Lin YP, Routledge SJ, Bawa Z, Knowles TJ, Overduin M, Dekker N, Dafforn TR, Bill RM, Poyner DR, Wheatley M. G-protein coupled receptor solubilization and purification for biophysical analysis and functional studies, in the total absence of detergent. Biosci Rep. 35 e00188.


Hwang PM, Cai F, Pineda-Sanabria SE, Corson DC, Sykes BD. The cardiac-specific N-terminal region of troponin I positions the regulatory domain of troponin C. Proc Natl Acad Sci USA. 111:14412-7.

Koppisetti RK, Fulcher YG, Jurkevich A, Prior SH, Xu J, Lenoir M, Overduin M, Van Doren SR. Ambidextrous binding of cell and membrane bilayers by soluble matrix metalloproteinase-12. Nature Commun. 5:5552.

Kufareva I, Lenoir M, Dancea F, Sridhar P, Raush E, Bissig C, Gruenberg J, Abagyan R, Overduin M. Discovery of novel membrane binding structures and functions. Biochem Cell Biol. 92:555-63.

Butterworth S, Overduin M, Barr AJ. Targeting protein tyrosine phosphatase SHP2 for therapeutic intervention. Future Med Chem. 6:1423-37.

Lenoir M, Sugawara M, Kaur J, Ball LJ, Overduin M. Structural insights into the activation of the RhoA GTPase by the lymphoid blast crisis (Lbc) oncoprotein. J Biol Chem. 289:23992-4004.




2001-2011 References



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