BioSpin - TopSpin
Dr. Clemens Anklin is a Vice President of Applications and Training for Bruker and an experienced user of the TopSpin software packaged that operates their spectrometers used to acquire high resolution multi-dimensional NMR data.
Dr. Clemens received his Ph.D. from the Swiss Federal Institute of Technology (ETH Zurich) and has been with Bruker since 1988. He presented at the first NMR BootCamp and also presented last year at the first "NANUC Roadshow" in London Ontario regarding non-uniform rapid NMR data acquisition on Bruker Instrumentation. His focus this year will be the set-up, acquisition and processing of BioNMR experiments. The lecture will also include a discussion of the latest additions to the experimental library and new features.
We are very pleased that he will be able to join us again in August and we look forward to his lively presentation style.
Jeff Hoch, Director
NMR Structural Biology Facility
University of Connecticut Health Center, CT, USA
Rowland NMR ToolKit (RNMRTK)
Jeff Hoch is a professor at the University of Connecticut who specializes in the processing of NMR data. His work specifically focuses on novel methods of data collection not limited to the use of Fourier Transformations.
NANUC was fortunate enough to have Prof. Hoch present at last year at the first offsite training course in London, Ontario, but the 2007 BootCamp will be Prof. Hoch's first BootCamp experience and the first time he will visit the national NMR centre in Edmonton (he has graciously agreed to try the straightjacket show at the Fringe Festival taking Wayne Fairbrother's place).
Prof. Hoch will be presenting the capabilities of the Rowland NMR ToolKit (commonly known as the RNMRTK) and will be demonstrating to students how to process rapid acquisition (i.e. non-uniform or non-linear) NMR data incompatible with traditional processing methods.
We eagerly look forward to his visit and latest developments.
Varian Inc. USA
VNMRJ and BioPack
Dr. Gray is a veteran of the Canadian national training courses here at NANUC and 2007 will be his second BootCamp and third invitation to NANUC. In 2001 Dr. Gray presented the predecessor to BioPack (then called ProteinPack which was later combined with RNAPack).
Dr. Gray will cover the newest version of the VNMRJ spectrometer software. Students will learn how to navigate the new system and utilize the various new user friendly components as well as perform customizations of their environments for individual requirements.
The demonstrations will then progress to the BioPack software suite of multi-nuclear and multi-dimensional pulse sequences. BioPack was designed to provide "User-friendly" water suppression, double and triple resonance 2D/3D/4D pulse sequences, including TROSY options and BioNMR PSG programming; featuring automatic calibration and experiment execution. It includes experiments for non-labeled and labeled biomolecules such as peptides, proteins, RNA and DNA's. Attendees will be able to utilize cutting edge pulse sequences from large contributing laboratories such as the Lewis Kay (Univ. of Toronto) Pulse sequence library.
Charles D. Schwieters
Head, Structural Biology Group NIH
Dr. Schwieters received his B.Sc. from the University of Virginia and his Ph.D. in Chemistry from Princeton.
He is involved in the planning and implementation of novel algorithms for probing protein structure and dynamics. He is responsible for the development and maintenance of the XPLOR-NIH and VMD-XPLOR software packages. He also supervises a small group at the main NIH campus.
2007 will be Charles' second NMR BootCamp and this year's focus will be on a more stepby-step approach to solving BioNMR problems with an emphasis on protein 3D structures and how to easily incorporate data and evaluate final solutions. Students will be presented with background information and then given several example problems to work on. Help will be provided and participants should leave with a basic understanding and ability to utilize the program to solve their research goals.
NMRPipe, NMRDraw, TALOS DYNAMO
Dr. Frank Delaglio is very familiar with the NANUC training courses having presented at a specialized course in 2003 and our first BootCamp during 2005. We of course enthusiastically included him in this years festivities and he has graciously volunteered to arrive early and stay late to answer students questions (you can also catch him earlier in the virtual world of "Second Life" as seen top image above).
Dr. Delaglio (aka the Big F. or the Big D.) will be presenting the extensive software suite from Prof. Ad Bax's laboratory at the National Institutes of Health. The software includes the well known and widely utilized NMRPipe, NMRDraw processing tools as well as the powerful TALOS, and DYNAMO programs. The emphasis this year will be on real world examples for students to work through.
As always we look forward to Frank's detailed yet relaxed lecture style.
Univ. of Alberta
Dr. Spyracopoulos is an Associate Professor in the Department of Biochemistry at the University of Alberta. The Spyracopoulos laboratory is involved in determining the dynamics, structures, and interactions of enzymes of the ubiquitination cycle, a biochemical process important in DNA repair and the immune response.
Dr. Spyracopoulos specializes in the analysis of protein dynamics through measurement of 15N NMR relaxation. He recently developed a suite of Mathematica (Wolfram Research) Notebooks for analysis of protein dynamics data which are available via his laboratories web site. The key features of the programs include ease of use, automatic visualization of data and results, and computer compatibility.
The aims of this afternoon workshop will be to familiarize students with analysis of protein main chain 15N dynamics using methods such as spectral density mapping and the "model-free" approach. The applicability of the methods with respect to spectral quality and sample conditions such as protein oligomerization will be discussed. Overall, students will learn how to carefully analyze protein main chain dynamics data, apply dynamics approaches to answer questions regarding biological function, and use dynamics analyses to complement protein structure determination.
Chenomx Inc. Edmonton Alberta
Dr. Pascal Mercier is another veteran of the NANUC workshops. He is a structural biochemist with a passion for developing mac software and making programs more user friendly and accessible. He originally got involved coding additions for the C-version of NMRView called the "Navigator".
Dr. Mercier received his degree from the Brian Sykes laboratory at the University of Alberta, and he has recently begun working for Chenomx Inc., Edmonton, Alberta. He is involved in developing software capable of automatically interpreting spectra and identifying metabolic markers from human body fluids.
He originally became interested in CYANA after discovering the speed that structures could be generated using the different calculation algorithms incorporated into CYANA. He has since written conversion scripts with GUIs (graphic user interfaces) in order to facilitate the incorporation of NMRView data more easily into the structure calculation program.
We are extremely fortunate to have Dr. Mercier join us for the afternoon and we are grateful to Chenomx for providing his expertise to our workshop.
President, One Moon Scientific
Bruce Johnson is Founder and President of One Moon Scientific, Inc. He is the creator of NMRView, one of the leading programs for the analysis and visualization of data on proteins and nucleic acids from Nuclear Magnetic Resonance spectrometers.
Prior to One Moon Scientific, Inc., Bruce was a Senior Research Fellow at Merck & Co., Inc. During his 15 year career at Merck he specialized in applying the methods of structural biology, molecular modelling, and cheminformatics to research in drug discovery. Bruce holds a BS degree from the University of Washington and a PhD from Duke University.
Presentations will include all the operational features of NMRViewJ (i.e. opening data, making assignments, picking NOEs for structure calculations, and exporting). Students will gain the ability to analyse spectra from multi-dimensional experiments and how to proceed access online resources for further individual progress.